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This paper presents large eddy simulation (LES) and experimental studies of the combustion process of ethanol/air mixture in an experimental optical HCCI engine. The fuel is injected to the intake port manifolds to generate uniform fuel/air mixture in the cylinder. Two different piston shapes, one with a flat disc and one with a square bowl, were employed to generate different in-cylinder turbulence and temperature field prior to auto-ignition. The aim of this study was to scrutinize the effect of in-cylinder turbulence on the temperature field and on the combustion process. The fuel tracer, acetone, is measured using laser induced fluorescence (LIF) to characterize the reaction fronts, and chemiluminescence images were recorded using a high speed camera, with a 0.25 crank angle degree resolution, to further illustrate the combustion process. Pressure in the cylinder is recorded in the experiments.