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Simulation of material circulation for a closed experiment using CEEF consisting of a plant and human system was performed. Initial set of materials contained in CEEF was decided by a decision procedure. Before the closure where the plant system is operated independently, the plant system (CPEF) needs gas exchange of O2 and CO2 with the outside. After the closure where the plant and human system are operated in a cooperation mode with mutual material exchange, no exchange of materials is needed. The closure time corresponds to the longest cultivation period to be cultivated in CPEF.