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An experimental study was performed to evaluate the effects of ethanol blending on to gasoline spray and combustion characteristics in a spray-guided direct-injection spark-ignition engine under lean stratified operation. The spray characteristics, including local homogeneity and phase distribution, were investigated by the planar laser-induced fluorescence and the planar Mie scattering method in a constant volume chamber. Therefore, the single cylinder engine was operated with pure gasoline, 85 %vol, 50 %vol and 25vol % ethanol blended with gasoline (E85, E50, E25) to investigate the combustion and exhaust emission characteristics. Ethanol was identified to have the potential of generating a more appropriate spray for internal combustion due to a higher vapor pressure at high temperature conditions. The planar laser-induced fluorescence image demonstrated that ethanol spray has a faster diffusion velocity and an enhanced local homogeneity.