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Hollow fiber membrane reactors (HFMBRs) may be used for biological wastewater treatment, and may be integrated with NASA's current research developments. The goal of this paper is to (a) evaluate the effect of mass transfer and hydrodynamics in a microporous HFMBR and (b) appropriateness of HFMBRs for use in space applications. Even though bubble-less aeration was not achieved by the use of microporous membranes, mass transfer within the HFMBR was found to increase after biofilm formation. Conversely, convective flow dominated transport within the system. Despite the high treatment efficiency obtained by the HFMBR, due to the bioreactor size, configuration and membrane spacing within the HFMBR, the bioreactor was not a suitable option for application under microgravity conditions. Even though developing a system with more favorable system hydrodynamics would aid in treatment efficiency, the use of a microporous HFMBR is not a recommended option to meet NASA's needs.

Hollow fiber membranes aerate wastewater without bubble formation by separating the liquid and gases phases with a semi-permeable membrane. These membranes have shown to successfully create aerobic conditions within a biological reactor. This research investigated the effect of long term usage and biofilm growth on membrane's ability to transfer oxygen to solution. Results show that oxygen transfer across the membrane decreased significantly compared to unused membranes in areas of high biofilm growth while low biofilm growth showed only slight decreases.

Microbial control in closed environmental systems, such as those of spacecraft or proposed base missions is typically limited to disinfection in the potable water system by a strong chemical agent such as iodine or chlorine. However, biofilm growth in the environmental system tubing threatens both the sterility of the potable water distribution as well as operational problems with wastewater systems. In terrestrial systems, biofilm has been recognized for its difficulty to control and eliminate as well as resulting operational problems. In order to maintain a potable water source for crew members as well as preventing operational problems in non-sterile systems, biofilm needs to be considered during system design. While biofilm controls can limit biofilm buildup, they are typically disruptive and cannot completely eliminate biofilm. Selenium coatings have shown to prevent initial biofilm attachment as well as limit attached growth on a variety of materials.

Biological pre-treatment of early planetary/lunar base wastewater has been extensively studied because of its predicted ability to offer equivalent system mass (ESM) savings for long term space habitation. Numerous biological systems and reactor types have been developed and tested for treatment of the generally unique waste streams associated with space exploration. In general, all systems have been designed to reduce organic carbon (OC) and convert organic nitrogen (ON) to nitrate and/or nitrite (NOx -). Some systems have also included removal of the oxidized N in order to reduce overall oxygen consumption and produce additional N2 gas for cabin use. Removal of organic carbon will generally reduce biofouling as well as reduce energy and consumable cost for physiochemical processors.

Bioreactor studies and microcosm experiments were conducted to determine the degradation potential of a commercial cleansing formulation. With the possible replacement of the current cleansing formulation under consideration (Ecolab whole body shampoo containing Igepon TC-42™ as an active ingredient), determination of the degradation characteristics of the alternative formulation is necessary. The commercial formulation currently being evaluated is a modified version of Pert Plus® for Kids (PPK). The degradation potential of the PPK and main surfactant Sodium Laureth Sulfate (SLES) was determined in a packed bed denitrifying bioreactor. Results from the bioreactor studies led to the development of stoichiometric relationships to help predict and monitor SLES degradation. In addition to the degradation rates of Ecolab, the PPK formulation, as well as the four leading constituents contained in the PPK formulation was determined under denitrifying conditions in microcosm studies.

When compared to physical and chemical processes for wastewater treatment in space, the benefits of biological systems include reduced storage and handling of waste material, lower energy requirements and plant growth system compatibility. An advanced membrane reactor (AMR) was constructed to treat ammonium-rich simulated wastewater. The effluent pH was approximately 6.3, and ammonium and TOC reduction rates were greater than 60 percent and 99 percent, respectively. The experimental results demonstrate that this technology may be suitable for space applications. However, the long-term performance of these systems should be investigated.

This research compared the nutrient content of the Biological Water Processor (BWP) effluent at JSC with acceptable nutrient ranges for general hydroponic NFT-solutions. Evaluated nutrient-components were NO3-N, P, K, Ca, Mg, Fe, Mn, Zn, B, Cu and Mo. Compared to Cooper's and Molyneaux's solution (Jones, 1997) BWP-nutrient concentrations were low for Ca, Mg, Fe and B. Compared to the NFT-solution used at KSC (Wheeler et al., 1997), the BWP-effluent showed higher contents of P, K, Zn, Cu and Mo and lower contents of N, Ca, Mg, Fe and B. This indicates that the BWP-effluent could support NFT-plant production.

This research is based on the hypothesis that recycling biofilm can provide the required carbon to increase biological denitrification of the carbon limited early planetary base wastewater. Recycling biofilm may offer significant advantages including a reduction in solid waste from biological wastewater processors, increased N2 return to cabin air, a reduction in TDS loading to the RO system, and increased alkalinity to drive further nitrification. The results of the study indicate that denitrification rate did increase due to the addition of lysed biofilm derived from the nitrification reactor. However, there was a simultaneous large release of additional ammonium. Further work will be required to understand the magnitude of the ammonium release and overall benefits of the process.

The purpose of this study was to evaluate the viability of treating wastewater through edible plant hydroponics. After the harvest in the hydroponic experiment (32 day study period), plant yield for edible biomass (corresponds to the harvested leaves) in wastewater and hydrosol (control) were 0.131 kg/m2 and 0.104 kg/m2, respectively. Potassium, TDS, and TN showed decreasing trends in hydrosol and wastewater during the experiment. Nitrification was observed in the wastewater unit with a significant increase (92.5%) in nitrate mass. Nitrite and ammonium mass in wastewater decreased with time, while hydrosol had negligible amounts of nitrite and ammonium during the study period. Calcium and magnesium masses decreased in the control and increased in wastewater. Wastewater showed a decrease in the mass of TOC (19.7%), while the hydrosol had negligible mass with respect to TOC.

The concept that plants and humans in a living system are mutually beneficial was communicated to 2nd - 12th grade students in science educational and outreach programs at Texas Tech University's Center for Space Science. Students traveled to the TTU horticulture greenhouse for a live program, which focused on research in the Engineering Development Unit. The research is funded by NASA's Advanced Life Support. During the program students were presented with the science of growing plants, how plants benefit humans in space, and baseline science vocabulary. A survey instrument was developed to assess student level of understanding of sciences, and their comprehension of living cycles, which work together to support manned space missions. The survey consisted of multiple-choice questions covering topics presented during the program. Likert questions were used to assess student's desire to travel in space, be an astronaut or a scientist, and their enjoyment of science and growing plants.

From 1997 to 2001, the third author worked with a team of engineers at JSC to develop the requirements and basic design for the Bioregenerative Planetary Life Support Systems Test Complex, or BIO-Plex. Under the Advanced Integration Matrix (AIM) Project, this earlier effort is extended to an investigation of methods and approaches for Advanced Systems Integration and Control. The intent is to understand and validate the use of software as an integrating function for complex heterogeneous systems, particularly for Advanced Life Support (ALS), in the context of support of mission operations. Preliminary investigations undertaken in the summer of 2004 indicate that integration of controls for the type of dynamic, non-linear, closed-loop biological systems under investigation for ALS systems require a different systems engineering approach than that required for traditional avionics systems.

A modified membrane-aerated biofilm reactor (mMABR) was constructed by incorporating two distinct biofilm immobilization media: gas-permeable hollow fiber membranes and high surface area inert bio-media. In order to evaluate the mMABR for space flight applications, a synthetic ersatz early planetary base (EPB) waste stream was supplied as influent to the reactor, and a liquid loading study was conducted at three influent flow rates. On average, percent carbon removal ranged from 90.7% to 93.1% with volumetric conversion rates ranging from 25 ± 3.3 g / m3 d and 95 ± 13.4 g / m3 d. Simultaneous nitrification/denitrification (SND) was achieved in a single reactor. As the liquid loading rate increased from 0.15 mL/min to 0.45 mL/min, the volumetric denitrification rates elevated from 27 ± 3.3 g / m3 d to 65 ± 5.2 g / m3 d. Additionally, it was found that nitrification and denitrification were linearly related with respect to both percent efficiency and volumetric reaction rates.