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A primary concern in creating a water reclamation system for long-duration manned space flight is the control of microbial contamination which can jeopardize water quality, compromise human health, and degrade constituent materials of the system. The microbial ecology facility in the Analytical and Physical Chemistry Branch of the Materials and Processes Laboratory at NASA's Marshall Space Flight Center (MSFC) is addressing this concern by means of experiments investigating the interaction of bacterial species in the development of a biofilm and their response to the introduction of additional species or to disinfection. Both static and recycling water systems are used. In static experiments, varied sequence and timing of species introduction in binary bacterial biofilms on 316L stainless steel elucidate the mechanisms involved in biofilm formation.