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An 84 day wheat crop was grown in the Variable Pressure Growth Chamber (VPGC) at Johnson Space Center (JSC). The VPGC is an atmospherically closed, controlled environment facility used to evaluate the use of higher plants as part of a regenerative life support system. The chamber has 10.6 m2 of growing area consisting of 480 pots of calcined clay support media. The chamber is lit by very high output, cool white fluorescent bulbs. Five wheat seeds were planted per pot giving a seeding density of 227 seeds·m-2. Pots were irrigated with a modified half strength Hoagland's nutrient solution three or six times per day depending on the crop age. At the plant canopy, the average temperature during the test was 22 ° C, relative humidity was maintained at 69%, CO2 concentration was 1000 ppm, photoperiod was continuous light, and the light intensity averaged 350 μmol·m-2·s-1.