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Recently, due to the concern on shortage in the supply of energy source as well as the greenhouse effect of engine exhaust, the use of renewable fuel, e.g., biodiesel, in the transportation sector is promoted. However, there are distinctions on both the physical and chemical properties between biodiesel and fossil diesel. These deviations in fuel property would result in different combustion characteristics and affect the performance of exhaust aftertreatment system, including the catalytic converter and the diesel particle filter. The purpose of this study is to investigate the effect of biodiesel on the performance and the durability of diesel particle filter. The biofuel adopted in this study was obtained by transesterification from methanol and spent edible oils, and fulfills the requirements specified in EN 14214 standard. All the tests were conducted in a heavy diesel engine (ISUZU 4JJ1E4N). Two studies were carried out in this paper.