Search Results

Three replicate aerobic-heterotrophic biotrickling filters were designed to promote the simultaneous biodegradation of graywater and a waste gas containing NH3, H2S and CO2. Upon visual observation of discolored solids, it was originally hypothesized that gas-phase CO2 concentrations were excessive, causing regions of anoxic zones to form within the biotrickling filters. Observed discolored (black) biofilm of this nature is typically assumed to be either lysed bacterial cells or anaerobic regions, implying alteration of operational conditions. Solid (biofilm) samples were collected in the presence and absence of gas-phase wastestream(s) to determine if the gas-phase contaminants were contributing to the solid-phase discoloration. Two sets of experiments (shaker flask and solids characterization) were conduced to determine the nature of the discolored solids. Results indicated that the discolored solids were neither anaerobic bacteria nor lysed cells.

Complete reuse of graywater will be essential during long duration human space missions. The highest loaded and most important component to remove from graywater is surfactant, the active ingredient in soaps and detergents. When considering a biological treatment system for processing of graywater, surfactant biodegradability becomes a very important consideration. Surfactants should be chosen that are degraded at a fast rate and yield inconsequential degradation byproducts. Experiments conducted for this research examined the biodegradation of the surfactants in Pert Plus for Kids, disodium cocoamphodiacetate (DSCADA) and sodium laureth-3 sulfate (SLES), using respirometry. Rates of CO2 production, or ultimate degradation, are reported. DSCADA was found to be toxic to bacteria when present at 270 ppm whereas no toxicity was observed during experiments with SLES.