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High-speed laser diagnostics was utilized for single-cycle resolved studies of the fuel distribution in the combustion chamber of a truck-size HCCI engine. A multi-YAG laser system consisting of four individual Nd:YAG lasers was used for planar laser-induced fluorescence (PLIF) imaging of the fuel distribution. The fundamental beam from the lasers at 1064 nm was frequency quadrupled in order to obtain laser pulses at 266 nm suitable for excitation of acetone that was used as fuel tracer. Bursts of up to eight pulses with very short time separation were produced, allowing PLIF images with high temporal resolution to be captured within one single cycle event. The system was used together with a high-speed framing camera employing eight ICCD modules, with a frame-rate matching the laser pulse repetition rate.