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Transgenic Plant Biomonitors: Stress Gene Biocompatibility Evaluation of the Plant Growth Facility for Pgim-01

Document Number: 2001-01-2181

Date Published: July 2001

Author(s):
Anna-Lisa Paul - Univ of Florida
Elizabeth Bihn - Univ of Florida
Chris Daugherty - Univ of Florida
Robert J. Ferl - Univ of Florida
David Chapman - Dynamac Corp
Kelly Norwood - Bionetics Corp

Abstract:
The initial task in the preparation for Flight Experiment PGIM-01 (Plant Growth in Microgravity - 01) was the optimization of the plant nutrient system within the environment of the Plant Growth Facility (PGF - a Space Shuttle middeck locker plant growth unit). PGIM-01 entailed using the Transgenic Arabidopsis Gene Expression System (TAGES) to monitor effects of spaceflight- associated stress on gene expression. TAGES plants are genetically engineered arabidopsis plants designed with a sensitive reporter gene system that responds to a variety of environmental stresses. However, transgene expression can also be influenced by background environmental conditions. Thus, minimizing sources of background stress on the plants was crucial to ensure optimal growth and a high scientific return.

File Size: 238K
Product Status: In Stock

See other papers presented at 31st International Conference On Environmental Systems, July 2001, Orlando, FL, USA, Session: Plant Flight Hardware: Key Technologies and Challenges

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