The use of water injection in a Homogeneous Charge Compression Ignition (HCCI) engine was experimentally investigated. The purpose of this study was to examine whether it is possible to control the ignition timing and slow down the rate of combustion with the use of water injection. The effects of different water flows, air/fuel ratios and inlet pressures were studied for three different fuels, iso-octane, ethanol and natural gas.It is possible to control the ignition timing in a narrow range with the use of water injection, but to the prize of an increase in the already high emissions of unburned hydrocarbons. The CO emission also increased. The NOx emissions, which are very low for HCCI, decreased even more when water injection was applied. The amount of water used was of the magnitude of the fuel flow.