Browse Publications Technical Papers 1999-01-2031

DNA Microchip-Based Microbial Montoring 1999-01-2031

Microbial monitoring of spacecraft and spacestations is crucial to maintaining system integrity and crew member health. Ribosomal RNA-based methods of microbial detection are particularly effective. A new DNA microchip technology now provides advantages over more traditional hybridization formats. This method utilizes a micro-array of gel elements containing hundreds of individual DNA-probes bound to a glass slide. The probes are complementary to the 16S rRNA of a selected bacterial species, genus, or higher taxonomic grouping. This format provides for massively parallel hybridization and the simultaneous identification of many microbial population types. We are developing a DNA microchip technology to monitor microbial contaminants of water systems


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