The Cambustion fast-response flame ionization detector (FFID) has been successfully used for instantaneous exhaust port hydrocarbon (HC) concentration measurement in IC engines for a decade. Measurements of in-cylinder HC concentration have also been made, but these present greater challenge. As the sample transit time and the time constant of the system always change when the sampling pressure is changed, it is necessary to investigate the characteristics of the system before it was used for in-cylinder sampling. A unique method was designed to study the influence of the diameter and length of the transfer sample line and the operating parameters of the FFID on the transit time and time constant. A database of transit time and time constant was built up for different simulated in-cylinder pressures. The database can be used for correcting eventual in-cylinder HC concentration measurement.