For a better understanding of the auto-ignition phenomenon in internal combustion engines, consideration is given from the in-cylinder gas temperature aspect. Experimental results demonstrate that the in-cylinder gas temperature at the end of compression, namely, the “auto-ignition temperature” is deeply involved in the onset of auto-ignition. The relation between the gas exchange state and the auto-ignition temperature explains the mechanism of timing controlled auto-ignition, namely, Activated Radical (AR) Combustion. The auto-ignition temperature is maintained constant during the AR combustion state, thanks to the exhaust valve controlling the hot residual gas amount. Finally, the utilization of auto-ignition in gasoline engines is discussed from the methodology aspect.