Molecular Labeling and Cell Detection of Food Pathogens on Food Processing Surfaces for ISS and Planetary Outpost 2003-01-2376
Food processing surfaces were cleaned and treated with low-levels of Listeria and Salmonella for surface swabbing. Swabs were placed in the appropriate enrichment broth for 6-18 hours. Immunomagnetic separation of the bacteria from the media was followed with Syto 62 fluorescent tagging and RBD2100 enumeration. Capture efficiencies for each organism were typically >80% and RBD2100 and standard plate counts correlations were >0.9 (R2). In a second method bacteria were centrifuged from the media, fixed, and hybridized with Cy-5 labeled rRNA specific probes. RBD analysis showed the presence of pathogen specific populations from the contaminated surface swabs.
Citation: Harkins, K., Harrigan, K., and Dickson, J., "Molecular Labeling and Cell Detection of Food Pathogens on Food Processing Surfaces for ISS and Planetary Outpost," SAE Technical Paper 2003-01-2376, 2003, https://doi.org/10.4271/2003-01-2376. Download Citation
Author(s):
Kristi R. Harkins, Kelley A. Harrigan, James S. Dickson
Affiliated:
Advanced Analytical Technologies, Inc. and NASA Food Technology Commercial Space Center Partner, Iowa State University and NASA Food Technology Commercial Space Center Affiliate
Pages: 11
Event:
International Conference On Environmental Systems
ISSN:
0148-7191
e-ISSN:
2688-3627
Related Topics:
Bacteria
Biological sciences
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