Q-PCR Based Bioburden Assessment of Drinking Water Throughout Treatment and Delivery to the International Space Station 2005-01-2932
Previous studies indicated evidence of opportunistic pathogens in samples obtained during missions to the International Space Station (ISS). This study utilized TaqMan quantitative PCR to determine specific gene abundance in potable and non-potable ISS waters. Probe and primer sets specific to the small subunit rRNA genes were designed and used to elucidate overall bacterial rRNA gene numbers. In addition, primer-probe sets specific for Burkholderia cepacia and Stenotrophomonas maltophilia were optimized and genes of these two opportunistic pathogens quantified in the pre- and post-flight drinking water as well as coolant waters. This Q-PCR approach supports findings of previous culture-based studies however; the culture based studies may have underestimated the microbial burden of ISS drinking water.
Citation: Newcombe, D., Stuecker, T., La Duc, M., and Venkateswaran, K., "Q-PCR Based Bioburden Assessment of Drinking Water Throughout Treatment and Delivery to the International Space Station," SAE Technical Paper 2005-01-2932, 2005, https://doi.org/10.4271/2005-01-2932. Download Citation
David Newcombe, Tara Stuecker, Myron La Duc, Kasthuri Venkateswaran
Jet Propulsion Laboratory, California Institute of Technology
International Conference On Environmental Systems