International Space Station Internal Active Thermal Control System: An Initial Assessment of the Microbial Communities within Fluid from Ground Support and Flight Hardware 2005-01-3094
The Internal Active Thermal Control System (IATCS) aboard the International Space Station (ISS) maintains appropriate ambient cabin temperatures. The microbial community of IATCS fluid was examined using conventional culture-based and advanced molecular techniques including adenosine triphosphate (ATP) and Limulus Amebocyte Lysate (LAL) assays, direct microscopic examination, and restriction fragment length polymorphism (RFLP) analyses of 16S rDNA genes from the community metagenome. About 50% of the samples examined showed populations that are uncultivable but contained viable cells. RFLP analyses identified nine distinct bacterial groups from the 171 clones and eight distinct bacterial groups from the 26 cultivable organisms,indicating significant diversity.
Citation: Benardini, J., Ballinger, J., Crawford, R., Roman, M. et al., "International Space Station Internal Active Thermal Control System: An Initial Assessment of the Microbial Communities within Fluid from Ground Support and Flight Hardware," SAE Technical Paper 2005-01-3094, 2005, https://doi.org/10.4271/2005-01-3094. Download Citation
James Benardini, Jordan Ballinger, Ronald L. Crawford, Monsi Roman, Randall Sumner, Kasthuri Venkateswaran
Environmental Biotechnology Institute, University of Idaho, Environmental Control and Life Support, Design and Development Branch, NASA Marshall Space Flight Center, The Bionetics Corporation, NASA Kennedy Space Center, Biotechnology and Planetary Protection Group, Jet Propulsion Laboratory, California Institute of Technology
International Conference On Environmental Systems
SAE 2005 Transactions Journal of Aerospace-V114-1