Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System 2006-01-2212
Single Loop for Cell Culture (SLCC) consists of individual, self-contained, spaceflight cell culture systems with capabilities for automated growth initiation, feeding, sub-culturing and sampling. The cells are grown and contained within a rigid cell specimen chamber (CSC). Bladder tanks provide flush and media fluid. SLCC uses active perfusion flow to provide nutrients and gas exchange, and to dilute waste products by expelling depleted media fluid into a waste bladder tank. The cells can be grown quiescently, or suspended using magnetically coupled stirrers. This paper describes the functional and safety design features, the operational modes and the spaceflight qualification processes including science validation tests, using yeast as a model organism.
Citation: Hoehn, A., Freeman, J., Koenig, P., Stodieck, L. et al., "Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System," SAE Technical Paper 2006-01-2212, 2006, https://doi.org/10.4271/2006-01-2212. Download Citation
Alexander Hoehn, Jacob Freeman, Paul Koenig, Louis Stodieck, Matthew Vellone, Shea Williams, Wendy Feenstra, Javier de Luis, Joe C. Parrish, Stephan Pretorius, Robert Renshaw, Liping Sun, Stephen Dyble, Nancy D. Searby, Donald Vandendriesche
BioServe Space Technologies, Aerospace Engineering Sciences, University of Colorado, Payload Systems Inc, NASA Ames Research Center
International Conference On Environmental Systems
SAE 2006 Transactions Journal of Aerospace-V115-1