Browse Publications Technical Papers 2006-01-2212
2006-07-17

Single Loop for Cell Culture (SLCC) – Development and Spaceflight Qualification of a Perfusion Cell Culture System 2006-01-2212

Single Loop for Cell Culture (SLCC) consists of individual, self-contained, spaceflight cell culture systems with capabilities for automated growth initiation, feeding, sub-culturing and sampling. The cells are grown and contained within a rigid cell specimen chamber (CSC). Bladder tanks provide flush and media fluid. SLCC uses active perfusion flow to provide nutrients and gas exchange, and to dilute waste products by expelling depleted media fluid into a waste bladder tank. The cells can be grown quiescently, or suspended using magnetically coupled stirrers. This paper describes the functional and safety design features, the operational modes and the spaceflight qualification processes including science validation tests, using yeast as a model organism.

SAE MOBILUS

Subscribers can view annotate, and download all of SAE's content. Learn More »

Access SAE MOBILUS »

Members save up to 18% off list price.
Login to see discount.
Special Offer: Download multiple Technical Papers each year? TechSelect is a cost-effective subscription option to select and download 12-100 full-text Technical Papers per year. Find more information here.
We also recommend:
TECHNICAL PAPER

Space Life Support from the Cellular Perspective

2001-01-2229

View Details

TECHNICAL PAPER

Design and Test of an Anaerobic Prototype Reactor Coupled with a Filtration Unit for the Liquefying Compartment of the MELiSSA Loop

2005-01-3067

View Details

TECHNICAL PAPER

High Pressure Water Electrolysis for the Space Station

871473

View Details

X