A diesel engine developed for an international market must be able to run on different fuels considering the diesel fuel qualities and the increasing selection of biofuels in the world. This leads to the question of how different fuels perform relative to a standard diesel fuel when not changing the hardware settings. In this study five fuels (Japanese diesel, MK3, EN590 with 10% RME, EN590 with 30% RME and pure RME) have been compared to a reference diesel fuel (Swedish MK1) when run on three different speeds and three different loads at each speed. The experiments are run on a Scania 13l Euro5 engine with standard settings for Swedish MK1 diesel. In general the differences were not large between the fuels. NO x usually increased compared to MK1 and then soot decreased as would be expected. The combustion efficiency increased with increased RME contents of the fuel but the indicated efficiency was not influenced by RME except for at higher loads. The pressure rise rates were increased compared to MK1, in most cases, for all fuels and especially for the Japanese fuel.