Structural adhesive joints are expected to retain integrity in their entire service-life that normally involves cyclic loading concurrent with environmental exposure. Under such a severe working condition, effective determination of fatigue life at different temperatures is crucial for reliable joint design. The main goal of this work was thus defined as evaluation of fatigue performance of adhesive joints at their extreme working temperatures in order to be compared with their fracture properties under static loading. A series of standard double-cantilever-beam (DCB) specimens have been bonded by three structural 3M epoxy adhesives selected from different applications. The specimens were tested under monotonic and cyclic opening loads (mode-I) in order to evaluate the quasi-static and fatigue performances of selected adhesives at room temperature, 80°C and -40°C. The test results revealed that the fatigue damage occurred at relatively low load levels when compared to quasi-static fracture forces. At room temperature, the maximum cyclic fatigue forces varied between 25% and 40% of corresponding quasi-static fracture loads of selected adhesives. More significant reductions in adhesive mechanical performances were observed at 80°C. At cryogenic temperature, the adhesives had their own characterizations; mainly increasing the fatigue resistance but very sensitive to testing parameters such as loading rate or crosshead speed. In conclusion, the experimental observations showed a significant influence of fatigue loading on adhesive joints mechanical performances that should be considered in joint design, particularly at non-ambient temperatures.