In a motorcycle gasoline engine, the port fuel injection system is rapidly spread. Compared to an automotive engine, the injected fuel does not impinge on the intake valve due to space restriction to install the injector. In addition, as the air flow inside the intake pipe may become very fast and has large cycle-to-cycle variation, it is not well found how the injector should be installed in the intake pipe to prepare “good” fuel-air mixture inside the intake pipe. In this study, the formation process of the fuel-air mixture is measured by using ILIDS system that is a 2-D droplets' size and velocity measurement system with high spatial resolution. Experiments with changing conditions such as flow speed and injection direction are carried out. As a result, the effects of injection direction, ambient flow speed and wall roughness on the fuel-air mixture formation process was examined, considering the three conditions of cold start, light to medium load operation and high load operation.