A measurement method for valve behavior during engine firing is established. In order to grasp valve behavior accurately, it has been required to develop a measurement method for valve behavior that takes in account for the condition during engine firing. However, behaviors of a valve train have generally been analyzed during engine motoring because it is difficult to measure them during engine firing. In this study, valve behavior during engine firing can be measured accurately by attaching a gap sensor to the valve guide. Furthermore, the simulation system for valve behavior that treated the valve train as three-dimensional flexible body is built. Under engine motoring condition, high correlation between measurement and simulation is confirmed for valve behavior and spring stress. Through this study, measurement of valve behavior during engine firing is established and under engine motoring condition, an optimal valve train system can be considered with high accuracy in the early stages of design.