Partially Premixed Combustion (PPC) is a combustion concept by which it is possible to get low smoke and NOx emissions simultaneously. PPC requires high EGR levels to extend the ignition delay so that air and fuel mix prior to combustion to a larger extent than with conventional diesel combustion. This paper investigates the operating region of single injection PPC for three different fuels; Diesel, low octane gasoline with similar characteristics as diesel and higher octane standard gasoline. Limits in emissions are defined and the highest load that fulfills these requirements is determined. The investigation shows the benefits of using high octane number fuel for Multi-Cylinder PPC. With high octane fuel the ignition delay is made longer and the operating region of single injection PPC can be extended significantly. Experiments are carried out on a multi-cylinder heavy-duty engine at low, medium and high speed.