Ultrasonic spot welding (USW) is a promising joining method for magnesium to steel to overcome the difficulties of fusion welding for these two materials with significant differences in melting temperatures. In a previous paper, the results of ultrasonic spot welding of magnesium to steel, with sonotrode engaged Mg piece, was presented. In this study, same material combination (0.8-mm-thick galvanized mild steel and 1.6-mm Mg AZ31B-H24) was used, but with sonotrode engaging steel piece. Various welding time, from 0.4 to 2.0 sec, were applied. Tensile lap-shear test, optical metallography, and scanning electron micrography were conducted for joint strength measurement and microstructural evaluation. The joint strength reached over 4.2 kN at 1.8 sec welding time. Mg-Zn eutectic was formed at the interface, indicating the interfacial temperature over 344°C. The study demonstrated USW to be a viable process for potential manufacturing of mixed-metal joints. The process is shown to be insensitive to metal arrangement of either the AZ31 or steel engaged with the sonotrode.