The latest trend world-wide, is to restrict the use of fossil fuels and replace them partially or totally by renewable fuels. In the present study, Jatropha methyl ester (JME) blended with tyre pyrolysis oil (TPO) was tested in a single cylinder, 4 stroke, air cooled, direct injection (DI) engine, to evaluate the performance and emissions characteristics. Four JMETPO blends, namely, JMETPO5, JMETPO10, JMETPO15 and JMETPO20 were used as fuel in the engine. The performance and emission results were analysed and compared with those of diesel operation. The results of the investigation indicate that the engine can run with JMETPO blends without any engine modification. The brake thermal efficiency of the engine fueled with the blends decreased marginally compared to that of diesel. The CO, HC and smoke emissions were found to be lower for the JMETPO blends in comparison with diesel. The NO emission increased with the JMETPO blends. The results are presented in this paper.