This paper introduces control strategy analysis and performance degradation for the 2010 Toyota Prius under different thermal conditions. The goal was to understand, in as much detail as possible, the impact of thermal conditions on component and vehicle performances by analyzing a number of test data obtained under different thermal conditions in the Advanced Powertrain Research Facility (APRF) at Argonne National Laboratory. A previous study analyzed the control behavior and performance under a normal ambient temperature; thus the first step in this study was to focus on the impact when the ambient temperature is cold or hot. Based on the analyzed results, thermal component models were developed in which the vehicle controller in the simulation was designed to mimic the control behavior when temperatures of the components are cold or hot. Further, the performance degradation of the components was applied to the mathematical models based on analysis of the test data. All the thermal component models were integrated into a vehicle system with the redesigned supervisory controller, and the vehicle model was validated with the test data. The validation results showed that fuel economies within 4% can be predicted, even when the ambient temperature is cold or hot.