This study investigated the effect of streamer discharge on autoignition and combustion in a Homogeneous Charge Compression Ignition (HCCI) engine. A continuous streamer discharge was generated in the center of the combustion chamber of a 2-stroke optically accessible engine that allowed visualization of the entire bore area. The experimental results showed that the flame was initiated and grew from the vicinity of the electrode under the application of a streamer discharge. Subsequently, rapid autoignition (HCCI combustion) occurred in the unburned mixture in the end zone, thus indicating that HCCI combustion was accomplished assisted by the streamer discharge. In other word, ignition timing of HCCI combustion was advanced after the streamer discharging process, and the initiation behavior of the combustion flame was made clear under that condition.