This paper investigates the scavenging process, in-cylinder gas motion in an opposed-piston two-stroke diesel engine and compares the results of in-cylinder gas motion to those of a uniflow-scavenged two stroke conventional engine using computational fluid dynamics engine models. The effect of piston motion profile of OP2S on the scavenging performance was discussed and its optimization was developed. Subsequently, CFD simulation on full load scavenging process was conducted at the same intake pressure and simulation at 2500rpm showed an optimum scavenging performance evaluated by delivery ratio, trapping efficiency and scavenging efficiency. Enhanced axial velocity and average turbulence kinetic energy around minimum volume center were found for OP2S diesel engine compared to the conventional two-stroke diesel engine. But the local turbulence kinetic energy at the areas where the injectors were located was lower instead, which meant the position of the injectors must be further optimized for OP2S.