Two-wheeled off-road vehicles are mainly ridden on slippery dirt roads that include steep slopes and rough, uneven surfaces. An analysis method for the driving state and the vehicle movement limits that would be suitable for analyzing the movement of such two-wheeled off-road vehicles under these conditions was examined. These movement limits were then formulated by taking into consideration the coefficient of friction and the road surface gradient in accordance with the basic laws of physics and also by focusing on the vehicle movement in the longitudinal direction. Measurements were also taken during actual off-road riding by top-class Japanese off-road motorcycle riders. It was confirmed that this measurement data was distributed within the range of the assumed vehicle movement limits. Consequently, it was confirmed that it is possible to use such measurements to accurately grasp the vehicle movement limits and the associated driving state for two-wheeled off-road vehicles.