Electronic shifter is an important human-machine interaction device in modern vehicles. To reduce the influence of shifting mechanism and structural parameters on control comfort, in the early stage of product quality design, we proposed a grey relation grade dynamic robustness method. In this paper, based on the multi-body dynamic model of gear shifting mechanism that was verified by experiments, the dynamic shifting force can be calculated by the parameterized multi-body dynamic model, and used as dynamic response variables. Then we stablished the linear relationship between response variables and signal factors, and measured the correlation degree between response variables and signal factors by the grey relation grade analysis. And choose the groove structure’s parameters, the contact parameters, the compression spring’s stiffness and preload as controllable factors. The influence of each controllable factors on the response signals determined by the variance analysis, and get the order of controllable factors ，then taking the controllable factors with significant effects as the optimal combination when the variance of the response variable is the minimum, finally obtained the robustness parameters of the control comfort of transmission shifting mechanism. The results of this paper can be used as a reference for engineers to design the new type of electronic shifting mechanism, which has strong engineering practicability.