Combustion studies on various potential alternative fuels were performed for the U.S. Array Belvoir Research and Development Center in a two-stroke heavy duty diesel engine. One cylinder of the engine was instrumented with a pressure transducer. A high-speed data acquisition system was used to acquire cylinder pressure histories synchronously with crankangle. The heat release diagrams, along with the calculated combustion efficiencies of the fuels were compared to a referee grade diesel fuel. The calculated and measured combustion parameters include heat release centroids, cumulative heat release, peak pressure, indicated horsepower, peak rate of pressure rise, indicated thermal efficiency, energy input, and ignition delay. Regression analyses were performed between various fuel properties and the calculated and measured combustion performance parameters. The fuel properties included specific gravity, cetane number, viscosity, boiling point distribution. H/C ratio, and net heat of combustion. A multiple linear regression was performed with the combustion parameters as the dependent variables and the fuel properties as the independent variables. The results of the heat release comparisons and the results of the regression analysis are discussed.