Current viscous drag reduction research explores the limits of practical applications of natural laminar flow (NLF) for airplane drag reduction. To better understand these limits, advanced measurement techniques are required to study the characteristics of laminar to turbulent boundary-layer transition. Recent NASA research indicates that the transition mode which involves laminar separation can be detected using arrayed hot-film laminar separation sensor concepts. These surface-mounted sensors can provide information on the location of the laminar separation bubble as well as bubble length. This paper presents two different laminar separation sensor configurations developed in the NASA program and presents results of wind-tunnel and flight evaluations of the sensors as tools to detect boundary-layer transition. Flight and wind-tunnel tests have shown the hot-film laminar separation sensor technique to be a viable means for detecting the existance of transition as well as Indicating reversed flow in a laminar separation bubble.