A Sequential fuel injection system was fitted to a 2 liter Nissan NAPS-Z engine that had been modified for neat methanol operation. The specific modifications for high compression operation with neat methanol are described, and baseline brake thermal efficiency and engine out emissions are established. Sequential injection operation on neat methanol included varying the beginning of injection between 50°BTDC and 250°ATDC over an equivalence ratio of 0.6 to 0.9. Efficiency and emission results with the Sequential system are compared to those from the base system and from selected references. For the low speed, steady state conditions used in this program, the Sequential system did not show any general improvement in efficiency or emissions. This result is directionally opposite to that observed in one reference. The apparent cause for the divergent results is the absence of mechanisms in this experiment to prevent mixing along the cylinder axis.