For human beings who have been reared on the earth with its 1 G gravitational field, the condition of weightlessness is a world with which we are unfamiliar. Even if the layout and equipment configuration of a spacecraft designed to compensate for operation under Zero-G conditions, there are some things which are not effective under actual weightless conditions. In the design of a manned spacecraft, it is necessary to accumulate design data on human performance in a weightless condition, then to undertake design evaluations and verification under weightless conditions. In this paper, testing for the purpose of evaluating the effectiveness of Zero-G simulation using neutral buoyancy, conducted first of all in Japan, and recommendations on the equipment and Facilities required to conduct such simulations, are described.