The purpose of this study is to experimentally investigate in-cylinder flows with cyclic variation in a practical part-loaded two-stroke engine. First, the in-cylinder LDV measurements are introduced, which were carried out above the port layout and the combustion chamber as well as the exhaust pipe or the transfer port together with the simultaneous pressure measurements. Second, the in-cylinder flow characteristics in different combustion groups were discussed. The in-cylinder flow and the combustion-chamber flow were not simply characterized by the pressure variation in the engine or the other passage flow in the exhaust pipe or the transfer port. Finally, the in-cylinder flow structure with three stages was shown using the vector variation analysis and the drawing of the velocity profiles in the engine parts.