Optical fiber probes were used to measure the soot temperature and estimate the soot concentration inside the cylinder of a DI diesel engine. The probes were mounted at various locations on the head of the test engine, and the measurements were performed under different load levels. Using the two-color method, the variations in temperature and soot mass concentration during the combustion process were examined with temporal and spatial resolution. It was observed that soot formation is rapid and is associated with heterogeneity in the early stage of combustion. Moreover, the soot formation mechanism seems to be independent of the engine load. In contrast, soot oxidation is relatively slow. Data obtained at several different load levels are presented, and the effects of various error sources on the accuracy of the measurement technique are also investigated.