To investigate NO formation in a combustion flame, PLIF (Planar Laser-Induced-Fluorescence) technique was applied to measure the NO fluorescence distribution in a constant-volume combustion chamber and in a sparkignition engine. The NO fluorescence distribution was taken by an image intensified CCD camera. In the constant-volume combustion chamber, the high NO fluorescence intensity was concentrically observed in the thin flame zone along the flame front. In postflame gas behind the flame zone, the NO fluorescence was widely distributed with weak intensity. In the case of the engine, the fluorescence was distributed in the broad flame zone. The fluorescence intensity had high value near the flame front, and decreased from the flame front to the postflame gas. As the equivalence ratio was changed, the fluorescence intensity reached maximum value at slightly lean condition. The mean NO fluorescence intensity at the end of combustion also had a peak in a slightly lean condition, and this tendency showed good correspondence with the characteristics of exhaust NOx emissions.