Three-dimensional numerical analysis of fuel liquid and mixture behavior in a port-injection gasoline engine is assessed by comparing calculations with measurements. The fuel mass distributed in the intake port and cylinder is measured using an engine with hydraulic valve and gas sampling system. The experimental results show that about half of the fuel mass per injection enters the cylinder, and the rest stays in the port. The difference of the mass fraction of injected fuel directly entering the cylinder is small between the cases of single pulse injection and serial injection. Therefore, three-dimensional calculation presupposing single pulse injection has difficulty in predicting the in-cylinder mixture formation process, although it can analyze the amount of fuel wetting the port wall. The calculations are performed for a port-injection engine, and the differences of fuel behavior with respect to swirl control valve opening and wall temperature are discussed.