One obstacle hindering the use of port fuel injection in natural gas engines is poor idle performance due to incomplete mixing of the cylinder charge prior to ignition. Fuel injection timing has a strong influence on the mixing process. The purpose of this work is to determine the impact of fuel injection timing on in-cylinder fuel distribution. Equivalence ratio maps have been acquired by Planar Laser Induced Fluorescence in an optical engine with a production cylinder head. Experimental results have been used to determine the injection timing which produces the most uniform fuel distribution for the given engine.