Modern engine management systems increasingly rely on on-line identification schemes. These are used either for self-tuning regulators or the rapid parametrization of controllers. In this paper the on-line parameter identification of the wall-wetting dynamics is studied in detail.The identification is performed by exciting the fuel path dynamics of the engine at a constant operating point. The amount of fuel injected serves as input and the air-to-fuel ratio, which is measured with a linear oxygen sensor, as output. In order to gain precise information about the amount of fuel in the cylinder, a new measurement concept is used. For one, the placement of the lambda sensor close to the exhaust valve minimizes the effects of gas mixing on the measurements. Additionally, by an appropriate collection of the data, the sensor dynamics are bypassed. This is also illustrated by a measurement with a very fast NOx sensor.With this new concept, frequency response measurements are produced which then are compared to several discrete model structures from literature. For the most suitable structure an on-line estimation algorithm is developed. Tests with measurement data from the engine show that the prediction error method, formulated for the case of the output error, is a viable approach.