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There are a few different ways in which biofuels can be sourced, with the most popular coming from agricultural sources. An alternative approach is to utilize biowaste. An estimated 20 million dry tons of volatile organic compounds, or biowaste, is annually deposited in US municipal wastewaters. Most of this biowaste energy content is not recovered and, as a result, the biowaste could be a massive potential source of renewable energy. Biocrude diesel is converted from wet biowaste via hydrothermal liquefaction (HTL). Three types of feedstocks (algae, swine manure, and food processing waste) were converted into biocrude oil via HTL. From the previous experiments done in an AVL 5402 single-cylinder diesel engine, it was observed that the presence of 20% of HTL in the blend performed similarly during combustion to pure diesel. By studying these mixtures in a constant volume chamber, these observations could be compared to the results in the diesel engine.