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The present paper describes some results of a cooperative research project between GM Powertrain Europe and Istituto Motori of CNR aimed at studying the impact of FAME and GTL fuel blends on the performance, emissions and fuel consumption of the latest-generation automotive diesel engines. The investigation was carried out on the newly released GM 2.0L 4-cylinder “torque-controlled” Euro 5 diesel engine for PC application and followed previous tests on its Euro 4 version, in order to track the interaction between the alternative fuels and the diesel engine, as the technology evolves. Various blends of first generation biodiesels (RME, SME) and GTL with a reference diesel fuel were tested, notably B20, B50 and B100. The tests were done in a wide range of engine operation points for the complete characterization of the biodiesels performance in the NEDC cycle, as well as in full load conditions.