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Proportional-integral-derivative (PID) controller is effective, popular and cost effective for a lot of scientific and engineering applications. In this paper, PID and fuzzy-self-tuning PID (FSTPID) controllers are applied to improve the performance of an active seat suspension system to enhance the pregnant woman comfort. The equations of motion of thirteen-degrees-of-freedom (13-DOF) active seat suspension system incorporating pregnant woman body model are derived and simulated. PID gains are tuned and estimated using genetic algorithm (GA) to formulate GA PID controller. In FSTPID, fuzzy logic technique is used to tune PID controller gains by selecting appropriate fuzzy rules using Matlab/Simulink software. Both controlled active seat suspension systems are compared with a passive seat suspension. Suspension performance is evaluated under bump and random road excitations in order to verify the success of the proposed controllers.