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It is necessary to develop a small positive pressure control system for the closed ecology experiment facility (CEEF) to protect against over-differential pressure loading. In the present study, a numerical method was developed to calculate the quantity of state of the closed module, which is fitted with rubber buffers, for the small positive pressure control system. Experiments to examine the pressure change of the closed module were carried out at CEEF. Comparison of calculated and experimental results showed that the present dynamic simulation is suited to estimating the quantity of state of the closed module.