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Direct-injection spark-ignition (DISI) engines have been adopted increasingly by the automotive industry in recent years due to their performance, reduced impact on the environment, and customer demand for advanced technology. However, detailed combustion processes in such engines are still not thoroughly analysed and understood. This work reports on the effects of different control parameters on the combustion process, such as fuel type, ignition timing and exhaust gas recirculation. Pure n-butanol and gasoline were used. All experiments were performed at 2000 rpm and 100 bar injection pressure in a transparent single-cylinder DISI engine equipped with the head of a commercial turbocharged engine with similar geometrical specifications (bore, stroke, compression ratio). Crank angle resolved 2D chemiluminescence in the UV range for OH radical and CO2 detection was performed with an ICCD camera and a high-speed CMOS camera was used for cycle resolved imaging.