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Knock is a factor hindering enhancement of the thermal efficiency of spark ignition engines, and is an unsteady phenomenon that does not necessarily occur each cycle. In addition, the heat release history of the flame also fluctuates from cycle to cycle, and the auto-ignition process of the unburned mixture (end-gas), compressed by the global increase in pressure due to release of chemical energy, is affected by this fluctuation. Regarding auto-ignition of the end-gas, which can be the origin of knock, this study focused on the fluctuation of the flame heat release pattern, and used a zero-dimensional (0D) detailed chemical reaction calculation in an attempt to analyze and examine the consequence on the end-gas compression and auto-ignition process of changes in the i) start of combustion, ii) combustion duration and iii) center of heat release of the flame.