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The Selective Catalytic Reduction (SCR) system installed on the exhaust line is currently widely used on Diesel heavy-duty trucks and it is considered a promising technique for light and medium duty trucks, large passenger cars and off-highway vehicles, to fulfill future emission legislation. Some vehicles of these last categories, equipped with SCR, have been already put on the market, not only in the US, where the emission legislation on Diesel vehicles is more restrictive, but also in Europe, demonstrating to be already compliant with the upcoming Euro 6. Moreover, new and more stringent emission regulations and homologation cycles are being proposed all over the world, with a consequent rapidly increasing interest for this technology. As a matter of fact, a physical model of the Diesel Exhaust Fluid (DEF) supply system is very useful, not only during the product development phase, but also for the implementation of the on-board real-time controller.