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Flight experiments are being developed to assess the microgravity performance of U.S.-developed physical/chemical life support technologies baselined for operation on the International Space Station (ISS). The experiments will take advantage of flight opportunities available on the Space Shuttle prior to the production of ISS flight systems. Early microgravity demonstrations of these technologies will allow the ISS life support system to be developed from flight-proven processes, thereby reducing programmatic risks and enhancing overall life support efficiencies. This paper will provide an overview of the life support flight experiment program.