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This paper presents a study of mixture formation in the combustion chamber of a direct-injection SI engine. In-cylinder flow measurement was conducted using laser Doppler velocimetry (LDV) and particle image velocimetry (PIV), and visualization of fuel vapor behavior was done using laser-induced fluorescence (LIF). Further, fast response flame ionization detector (FID) was used to measure the hydrocarbon (HC) concentrations in the vicinity of the spark plug. Thereby mixture concentrations in the vicinity of the spark plug, within the mixture distribution observed using LIF, were quantified. Results revealed that an upward flow forms near the center of the cylinder in the latter half of the compression stroke and goes from the piston crown toward the cylinder head. This upward flow is caused by the synergistic effect of the swirl motion generated in the cylinder and the cylindrical bowl provided in the piston crown eccentrically to the central axis of the cylinder.